Isolation and molecular identification of fungi contaminating fruits and vegetables in cold storages in Al – Anbar city and studying the effect of aqueous extract and dry powder of Eugenia caryophyllata on it.

The current study was conducted in the Fungi Research Laboratory, Department of biology, College Sciences, University Anbar , included collecting 93 samples infected fruits (fruits and vegetables) Cold stores for different regions Al- province. fungi that contaminated were isolated characterized microscopically molecularly, aqueous plant extracts dry powders prepared Eugenia flowers purpose studying their effect on radial growth pathogenicity these fungi, Aspergillus flavus و niger ، Neurospora crassa This done by using method poisoning food medium PDA with concentration 10%, 20%,30% v/v extract 0.1, 0.2, 0.3 mg/ml powder powder, to evaluate efficacy against fungi. fungicidal Topsin M 70% also tested 0.02 PDA. active chemical compounds have been detected several reagents GC MASS technology pH value determined also.The results showed inhibited niger, flavus, Neurosporo Where highest inhibition percentage reached when 30% gave 6, 5.6 mm respectivily ratio 85.7, 93.4 % significant differences from control treatment at probability level 0.05.Also showed, it an effective towards A. A.flavus, N. crassa. ability stimulate resistance tomato before after infection incubation temperature 11º c. Pre-inoculated beyond. superiority pre-inoculation reducing severity fungus, as effectiveness N.crassa, amounting 26.6%, compared treatment, which infiction 80%.The qualitative detection indicated extract, contain many antifungal activity such alkaloids, resins, sugars, tannins, saponins, glycosides, Terpenes, flavonoids fucoumarins.GC-MS contains are attributed activity, euginol thymoquinone.